Anti-LAMP1 Monoclonal Antibody

Product Name

Anti-LAMP1 Monoclonal Antibody

View all LAMP-1/CD107a Antibodies

SKU/Catalog Number

M00780-1

Size

100ug

Form

liquid

Description

Boster Bio Anti-LAMP1 Monoclonal Antibody catalog # M00780-1. Tested in IP, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-LAMP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00780-1)

Host

Mouse

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

Ly1C6

Isotype

IgG1

Immunogen

Rat liver lysosomal membrane preparations

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~120kDa.

Reactive Species

M00780-1 is reactive to LAMP1 in Hamster, Human, Mouse, Rat

Applications

M00780-1 is guaranteed for IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

44.882kDa

Background of LAMP-1/CD107a

Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37% sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAMPCali, San Fran School of Medicine.

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Figure 2. Western blot analysis of Lamp1 using anti-Lamp1 antibody (M00780-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamp1 antigen affinity purified polyclonal antibody (Catalog # M00780-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for Lamp1.

Gene/Protein Information For LAMP1 (Source: Uniprot.org, NCBI)

Alternative Names

CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1 LAMP1 CD107a, LAMPA, LGP120 lysosomal associated membrane protein 1 lysosome-associated membrane glycoprotein 1|CD107 antigen-like family member A|LAMP-1|lysosome-associated membrane protein 1

For more info on LAMP1, check out the LAMP1 Infographic

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In this infographic, you will see the following information for LAMP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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